N-glycan (asialo-, mono-galactosylated biantennary) labeled with 2-aminobenzamide (2-AB), for use as a qualitative standard in applications such as liquid chromatography (LC). N-Glycans Standards. Agilent unlabeled N-glycan (formerly ProZyme) standards are used as qualitative standards for LC/FLD, LC/MS, or CE/LIF. AdvanceBio Man3 / M3 N-glycan standard (formerly ProZyme). listing masses of adduct ions of unlabeled and labeled glycans for mass spectrometry work. Glycan Standards Structures Technical Flyer, Critical Quality Attributes application compendium. Web Site Use Guidelines | Unlabeled standards offer flexibility in workflows using reductive amination labeling chemistry. Part Number: GKR-002300. … HAYWARD, Calif., Nov. 14, 2016 /PRNewswire-iReach/ -- ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-Q Glycan Analysis System, an integrated platform for high-throughput, user-friendly glycoanalysis. N-glycan (conserved trimannosyl core). O-Glycan Standards Featured Best Selling Alphabetically: A-Z Alphabetically: Z-A Price: Low to High Price: High to Low Date: New to Old Date: Old to New O-Glycans are N-acetyl-galactosamine linked glycans which can have a variety of core structures. Founded in 1990, ProZyme is an industry leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. Part Number:GKR-002300. Bioz Stars score: 88/100, based on 4 PubMed citations. Given the stoichiometry of the Schiff base and activated carbamate labelling reactions, each glycan can get only one fluorophore and direct relative quantitation by HPLC-FLD is possible. Nomenclature Committee, Consortium for Functional Glycomics; [2012; cited 2015 Oct 6]. Push your Glycoprotein analysis to new levels of performance. Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called glycans, is a fast-growing and important field of study in life sciences. The elucidated N-glycan structures are shown above each peak using the conventional glycan nomenclature of the Consortium for Functional Glycomics. The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC. Labeling of Standards • Individual glycan standards purchased from ProZyme show Founded in 1990, ProZyme is an industry-leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. Hayward, CA. Agilent Biocolumns - Critical Quality Attributes - Application Compendium. peak retention time in comparison to known standards, and the corresponding m/z value as deter-mined by ESI-MS, in agreement with the known metabolic pathways of antibody producing expression systems. AdvanceBio 2-AA G0 / A2 N-glycan standard (formerly ProZyme). Symbol and Text Nomenclature for Representation of Glycan Structure. Privacy Policy, For glycan masses, see the product pages and our. Alternative glycan names: NGA2. We offer an extensive range of AdvanceBio (formerly ProZyme) glycan standards and libraries. Also available are the GKLB-002 Bovine Fetuin N-linked Glycan Library (GKSB-002 for 2-AB-labeled library) and the GKLB-005 Human IgG N-linked Glycan Library (GKSB-005 for Careers | The company's focus on R&D and innovation supports an agile product development program... Read more Each batch of ACQUITY UPLC BEH Glycan column packing material is physically, chemically, and chromatography tested using a well characterized 2AB-labeled glycan standard. The Glycan Performance Test Standard line is intended to be used as a benchmarking standard for ACQUITY UPLC BEH Glycan, 1.7 µm Columns. Glycan Release • The Protein Express 200 assay was run on the LabChip platform as a control to test for the level of deglycosylation. Glycan Performance Test Standard . Certificates of Analysis | Add to Favorites. The GlycoClean™ S Cartridges were purchased from ProZyme® (Hayward, … Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called glycans, is a fast-growing and important field of study in life sciences. 15 Reductive Under reducing conditions there is a complete shift from the from the heavy chain (HC) to the non-glycosylation heavy chain (NGHC). Choose labeled or unlabeled standards. Blackrock, Ireland: National Institute for Bioprocessing Research and Training (NIBRT); c2012. Contact Us, Ordering Info | Events & Trade Shows | Glycobiology, which is the study of the structure, function and biology of carbohydrates, also called glycans, is a fast-growing and important field of study in life sciences. We offer an extensive range of AdvanceBio (formerly ProZyme) glycan standards and libraries. Alternative glycan names: NGA2. [Internet]. Average salaries for ProZyme Quality Control Associate: $24. Characterize your samples with confidence using highly purified glycan standards. Characterize your samples with confidence using highly purified glycan standards. AdvanceBio 2-AB G1 / A2G1 N-glycan standard (formerly ProZyme). Services : Data analysis will be simplified by the use of customized software and ProZyme N-glycan reference standards. Available from: CFG: Functional Glycomics Gateway [Internet]. The Glyko® 2-AB Human IgG N-Linked Glycan Library was purchased from ProZyme® (Hayward, CA), and the 2-AB labeled IgG N-Glycan Library was purchased from Ludger Ltd (Oxfordshire, U.K.). ProZyme salary trends based on salaries posted anonymously by ProZyme employees. The negative mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and APTS. Alternative glycan names: F … ProZyme is a fast-growing life sciences company that supplies value-added kits, enzymes, standards and complete systems for biotherapeutic protein characterization and glycobiology research. The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC. GlycoBase 3.2.4. Use glycan standards for liquid chromatography and capillary electrophoresis separations with fluorescence or mass spectrometry detection. Applications : The endoglycosi-dase digestion using PNGase F was performed at 50 °C for 1 h, followed by capture of the released glycans by the CleanSeq magnetic beads using 87.5% acetonitrile medium. About Prozyme : The positive mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and InstantPC. Qualitative standard for glycan identification. Global Distributors | Glyko® 2-AB A1F (G2FS1) [GKSB-315] $ Inquire. Product Commercialization Scientist ProZyme, Inc. 2008 – 2014 6 years. For glycan masses, see the product pages and our downloadable Excel file listing masses of adduct ions of unlabeled and labeled glycans for mass spectrometry work. GKC-004301. For glycan masses, see the product pages and our downloadable Excel file listing masses of adduct ions of unlabeled and labeled glycans for mass spectrometry work. One of the main CQA requirements for glycoproteins is relative glycan quantitation, i.e. Guide to the abbreviations used to identify 2AB labelled N-glycans; [cited 2016 Apr 19]. Founded in 1990, ProZyme is an industry leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. Glyko® 2-AA Human α1-Acid Glycoprotein N-Linked Glycan Library [GKSA-001] $ Inquire. SANTA CLARA, Calif.--(BUSINESS WIRE)-- Agilent Technologies Inc. (NYSE:A) today announced that it has completed the acquisition of ProZyme, Inc., a leading provider of glycan reagents, kits and standards.This acquisition expands Agilent’s portfolio of biopharma consumables. For more details on our glycan standards, or to request a new glycan standard product, please contact us. Subscribe to our newsletter and always be the first to hear about what is happening. News | AdvanceBio 2-AB G0 / A2 N-glycan standard (formerly ProZyme). Add to Favorites. N-glycan (asialo, agalacto biantennary complex) labeled with 2-aminobenzoic acid (2-AA, also known as anthranilic acid), for use as a qualitative standard in applications such as liquid chromatography (LC). N-glycan (asialo-, agalacto-, biantennary complex) labeled with 2-aminobenzamide (2-AB), for use as a qualitative standard in applications such as liquid chromatography (LC). GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans. Founded in 1990, ProZyme is an industry-leading developer and manufacturer of glycan reagents, kits and standards, which are required for efficient sample prep in the analysis of free glycans. Available from: Ceroni A, Maass K, Geyer H, Geyer R, Dell A, Haslam SM. PhycoLink Conjugates & Activated Phycobiliproteins, N-Glycan Identification and Characterization, N-Glycan Remodeling and Glycovariant Production, www.glycobase.nibrt.ie/glycobase/documents/abbreviations.pdf, http://glycomics.scripps.edu/CFGnomenclature.pdf, CHO mAb N-Glycan Library + CHO mAb Glycoprotein, Human α1-acid glycoprotein N-Glycan Library, Biantennary & High Mannose Partitioned Library, α(2-3) Sialylated Biantennary N-Glycan Library, α(2-6) Sialylated Biantennary N-Glycan Library, α(2-3) Sialylated Triantennary N-Glycan Library, α(2-6) Sialylated Triantennary N-Glycan Library, α(2-3) Sialylated Tetraantennary N-Glycan Library, α(2-6) Sialylated Tetraantennary N-Glycan Library, Internal Migration Standards for Capillary Electrophoresis (CE). Two versions of labeled standards for 2‑AB (2‑aminobenzamide and RapiFluor-MS; Human IgG based; QC verified to contain the components needed to benchmark and evaluate HILIC columns Substrates for EDGE profiling experiments were either individual N-glycan standards (Prozyme) or N-glycans released from polyclonal human IgG (MilliporeSigma). Founded in 1990, Hayward-Calif. based ProZyme is an industry leading developer and manufacturer of glycan … Qualitative standard for glycan identification. AdvanceBio G0F / FA2 N-glycan standard (formerly ProZyme). At the front end of the Gly-Q system, enzymatically-released and fluorescently-labeled glycans will be prepared using ProZyme chemistry prior to glycan separation using the automated CGE instrument. is correlated to glycan structures that have been previously determined. An assortment of standards and libraries are available. Legal | The entire workflow of the rapid and automated glycan sample preparation protocol is shown in Figure 3. Use glycan standards for liquid chromatography and capillary electrophoresis separations with fluorescence or mass spectrometry detection. N-glycan (asialo-, agalacto-, biantennary complex N-glycan, core-substituted with fucose). Alternatively, if a mixture of known standards is available, these standards can be analyzed under the same chromatographic conditions as the samples. The negative mode tab shows glycans labeled by 2-AB, InstantAB, 2-AA, Procainamide and APTS. Alternative glycan names: M3N2, MAN-3. Resources : determining the percentage of each glycan species. Terms of Use | corresponding ProZyme glycan standards (product codes GKC-124300, GKC-224300 and GKC-335300, respectively) with the GKK-404 Signal™ 2-AB Labeling Kit. Products : ZERO BIAS - scores, article reviews, protocol conditions and more Part Number: GKSB-301. Choose labeled or unlabeled standards. Once potential structures are identified, they are confirmed with a series of exoglycosidase digestions. 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